Reaction of N-(3-Pyrene)maleimide with Thiol Groups of Reticulocyte Ribosomes
نویسندگان
چکیده
منابع مشابه
The reaction of protein thiol groups with some disulphides.
Rapport, D. & Canzanelli, A. (1950). Science, 112, 469. Roffo, A. & Correa, L. (1938). Rev. sudamer. Endocr. 20, 548. Schmidt, G. & Thannhauser, S. J. (1945). J. biol. Chem. 161, 83. Schneider, W. C. (1945). J. biol. Chem. 161, 293. Smith, I. (1958). Chromatographic and Electrophoretic Technique8. London: William Heinemann (Medical Books) Ltd. Spiegel-Adolf, M. (1935). J. Amer. chem. Soc. 57, 1...
متن کاملGlobin synthesis on reticulocyte membrane-bound ribosomes.
Evidence is presented that indicates that greater than 95 % of the protein synthesized by intact rabbit reticulocytes is globin and that only globin nascent chains are found on the membrane-bound ribosomes from reticulocytes. This result conflicts with reports in the literature that approximately 14% of the protein synthesized by reticulocytes is non-globin and that 86% of the protein synthesiz...
متن کاملA study of the effects of reaction with formaldehyde on some optical and physical properties of reticulocyte ribosomes.
1. The optical rotatory dispersion and ultraviolet-absorption spectrum of ribosomal RNA in situ appear to be unchanged when the ribosome is dissociated into its RNA and protein moieties. 2. Reaction with 0.05% formaldehyde at 20 degrees for 2hr. ;fixes' ribosomes so that they remain intact in 1% sodium dodecyl sulphate. 3. The RNA moiety of the ribosome undergoes a conformational change when ri...
متن کاملInteraction of rabbit reticulocyte ribosomes with bacteriophage f1 mRNA and of Escherichia coli ribosomes with rabbit globin mRNA.
We have compared the behavior of a prokaryotic mRNA in a eukaryotic ribosome binding system and of a eukaryotic mRNA in a prokaryotic ribosome binding system. Using (32)P- and (125)I-labeled bacteriophage f1 mRNA, we have shown that rabbit reticulocyte 80S ribosomes can protect specific sequences from pancreatic RNase digestion, including those sequences protected by Escherichia coli ribosomes....
متن کاملThe production of biologically active subparticles from rabbit reticulocyte ribosomes.
THE EFFECT OF EXPOSING RABBIT RETICULOCYTE RIBOSOMES TO CONCENTRATED SOLUTIONS OF POTASSIUM CHLORIDE WAS EXAMINED BY: (a) dialysis against 0.5m-potassium chloride; (b) zone centrifugation through a sucrose gradient in 0.5m-potassium chloride; (c) differential centrifugation of a solution made 0.5m with respect to potassium chloride. The products of each treatment and their ability to support pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1976
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1976.tb10430.x